Warning
This repository has been archived as of 2026-01-12. The functionality has been migrated and expanded upon in the WILDS WDL Library as the ww-leukemia pipeline. Please refer to that instance for the latest versions, documentation, and updates.
This WILDS WDL workflow takes samples through a standard alignment protocol and identifies variants using a trio of well-established variant callers. Finally, the workflow narrows it down to variants of confidence by analyzing consensus amongst the three variant callers.
For Fred Hutch users that are new to WDL, we recommend using PROOF to submit this workflow directly to the on-premise HPC cluster, as it simplifies interaction with Cromwell and provides a user-friendly front-end for job submission and tracking. For users outside of Fred Hutch or more advanced users who would like to run the workflow locally, command line execution is relatively straightforward:
java -jar cromwell-86.jar run ww-vc-trio.wdl \
--inputs ww-vc-trio-inputs.json \
--options ww-vc-trio-options.json
Although Cromwell is demonstrated here, this pipeline is not specific to Cromwell and can be run using whichever WDL execution method you prefer (miniwdl, Terra, HealthOmics, etc.).
For questions, bugs, and/or feature requests, reach out to the Fred Hutch Data Science Lab (DaSL) at wilds@fredhutch.org, or open an issue on our issue tracker.
If you would like to contribute to this WILDS WDL workflow, see our contribution guidelines as well out our WILDS Contributor Guide for more details.
Distributed under the MIT License. See LICENSE for details.