Data and analysis code for Massively parallel protein-protein interaction measurement by sequencing (MP3-seq) enables rapid screening of protein heterodimers
##Contents:
- benchmarking: Code for comparison of MP3-Seq to benchmark datasets from the paper (Figures 2-3)
- data: Barcode counts for libraries used in the paper (both older and final MP3-Seq method versions)
- design_screening: DUET analysis code for large designed binder screens, and orthogonality gap scripts
- processing_pipeline: MP3-Seq processing pipeline with autoactivator screening, autoactivator correction, psuedoreplication, and R scripts to run DESeq2
- supplementary_analysis: Misc. scripts to replicate supplementary figures in the paper.
- Alphafold_and_Rosetta_metrics: Scripts to calculate ALphaFold and Rosetta metrics