diff --git a/src/libraries/System.Net.Http/src/System/Net/Http/SocketsHttpHandler/Http3RequestStream.cs b/src/libraries/System.Net.Http/src/System/Net/Http/SocketsHttpHandler/Http3RequestStream.cs
index 45b74472b402cb..7497ac2614e2ff 100644
--- a/src/libraries/System.Net.Http/src/System/Net/Http/SocketsHttpHandler/Http3RequestStream.cs
+++ b/src/libraries/System.Net.Http/src/System/Net/Http/SocketsHttpHandler/Http3RequestStream.cs
@@ -1142,6 +1142,11 @@ private async ValueTask<bool> ReadNextDataFrameAsync(HttpResponseMessage respons
                 switch (frameType)
                 {
                     case Http3FrameType.Data:
+                        // Ignore DATA frames with 0 length.
+                        if (payloadLength == 0)
+                        {
+                            continue;
+                        }
                         _responseDataPayloadRemaining = payloadLength;
                         return true;
                     case Http3FrameType.Headers:
diff --git a/src/libraries/System.Net.Http/tests/FunctionalTests/HttpClientHandlerTest.Http3.cs b/src/libraries/System.Net.Http/tests/FunctionalTests/HttpClientHandlerTest.Http3.cs
index df5b59b500eb37..653e1ead3360aa 100644
--- a/src/libraries/System.Net.Http/tests/FunctionalTests/HttpClientHandlerTest.Http3.cs
+++ b/src/libraries/System.Net.Http/tests/FunctionalTests/HttpClientHandlerTest.Http3.cs
@@ -380,7 +380,6 @@ public async Task ServerCertificateCustomValidationCallback_Succeeds()
         [OuterLoop]
         [ConditionalTheory(nameof(IsMsQuicSupported))]
         [MemberData(nameof(InteropUris))]
-        [ActiveIssue("https://github.com/dotnet/runtime/issues/54726")]
         public async Task Public_Interop_ExactVersion_Success(string uri)
         {
             if (UseQuicImplementationProvider == QuicImplementationProviders.Mock)
@@ -402,10 +401,36 @@ public async Task Public_Interop_ExactVersion_Success(string uri)
             Assert.Equal(3, response.Version.Major);
         }
 
+        [OuterLoop]
+        [ConditionalTheory(nameof(IsMsQuicSupported))]
+        [MemberData(nameof(InteropUrisWithContent))]
+        public async Task Public_Interop_ExactVersion_BufferContent_Success(string uri)
+        {
+            if (UseQuicImplementationProvider == QuicImplementationProviders.Mock)
+            {
+                return;
+            }
+
+            using HttpClient client = CreateHttpClient();
+            using HttpRequestMessage request = new HttpRequestMessage
+            {
+                Method = HttpMethod.Get,
+                RequestUri = new Uri(uri, UriKind.Absolute),
+                Version = HttpVersion.Version30,
+                VersionPolicy = HttpVersionPolicy.RequestVersionExact
+            };
+            using HttpResponseMessage response = await client.SendAsync(request, HttpCompletionOption.ResponseContentRead).WaitAsync(TimeSpan.FromSeconds(20));
+
+            Assert.Equal(HttpStatusCode.OK, response.StatusCode);
+            Assert.Equal(3, response.Version.Major);
+
+            var content = await response.Content.ReadAsStringAsync();
+            Assert.NotEmpty(content);
+        }
+
         [OuterLoop]
         [ConditionalTheory(nameof(IsMsQuicSupported))]
         [MemberData(nameof(InteropUris))]
-        [ActiveIssue("https://github.com/dotnet/runtime/issues/54726")]
         public async Task Public_Interop_Upgrade_Success(string uri)
         {
             if (UseQuicImplementationProvider == QuicImplementationProviders.Mock)
@@ -413,7 +438,9 @@ public async Task Public_Interop_Upgrade_Success(string uri)
                 return;
             }
 
-            using HttpClient client = CreateHttpClient();
+            // Create the handler manually without passing in useVersion = Http3 to avoid using VersionHttpClientHandler,
+            // because it overrides VersionPolicy on each request with RequestVersionExact (bypassing Alt-Svc code path completely).
+            using HttpClient client = CreateHttpClient(CreateHttpClientHandler(quicImplementationProvider: UseQuicImplementationProvider));
 
             // First request uses HTTP/1 or HTTP/2 and receives an Alt-Svc either by header or (with HTTP/2) by frame.
 
@@ -443,7 +470,7 @@ public async Task Public_Interop_Upgrade_Success(string uri)
                 using HttpResponseMessage responseB = await client.SendAsync(requestB).WaitAsync(TimeSpan.FromSeconds(20));
 
                 Assert.Equal(HttpStatusCode.OK, responseB.StatusCode);
-                Assert.NotEqual(3, responseB.Version.Major);
+                Assert.Equal(3, responseB.Version.Major);
             }
         }
 
@@ -754,14 +781,26 @@ private SslApplicationProtocol ExtractMsQuicNegotiatedAlpn(Http3LoopbackConnecti
 
         /// <summary>
         /// These are public interop test servers for various QUIC and HTTP/3 implementations,
-        /// taken from https://github.com/quicwg/base-drafts/wiki/Implementations
+        /// taken from https://github.com/quicwg/base-drafts/wiki/Implementations and https://bagder.github.io/HTTP3-test/.
         /// </summary>
         public static TheoryData<string> InteropUris() =>
             new TheoryData<string>
             {
-                { "https://quic.rocks:4433/" }, // Chromium
-                { "https://http3-test.litespeedtech.com:4433/" }, // LiteSpeed
-                { "https://quic.tech:8443/" } // Cloudflare
+                { "https://www.litespeedtech.com/" }, // LiteSpeed
+                { "https://quic.tech:8443/" }, // Cloudflare
+                { "https://quic.aiortc.org:443/" }, // aioquic
+                { "https://h2o.examp1e.net/" } // h2o/quicly
+            };
+
+        /// <summary>
+        /// These are public interop test servers for various QUIC and HTTP/3 implementations,
+        /// taken from https://github.com/quicwg/base-drafts/wiki/Implementations and https://bagder.github.io/HTTP3-test/.
+        /// </summary>
+        public static TheoryData<string> InteropUrisWithContent() =>
+            new TheoryData<string>
+            {
+                { "https://cloudflare-quic.com/" }, // Cloudflare with content
+                { "https://pgjones.dev/" }, // aioquic with content
             };
     }
 }